推荐最近最火的赌博软件/Leica Center Of Excellence For Advanced Light Microscopy

http://www.Upstate.edu/neuroscience-department/research/confocal.php

1. STED(受激发射耗尽)3X纳米显微镜
   This is a super-resolution technique that overcomes diffraction limited resolution. 有多个波长为592 nm的STED激光线, 660nm和脉冲激光在775nm, STED可以达到30纳米以下的分辨率.
   主要特点:
   • 紫外激光器405 nm，白光激光器WLL2 470-670 nm.
   • STED激光器:592 nm, 660 nm和775 nm.
   • 测试物镜:93倍甘油和100倍油.
   • Five spectral detector channels: two PMTs and three HyDs.
   • 串联扫描器(8kHz).
   • XY扫描台.
   • 用于STED的HyVolution2.
2. 数字光片(DLS)显微镜
   DLS microscopy is a fluorescence technique that uses a plane of light for 3-D imaging that is useful for large samples such as embryos, 敏感的样品, 或者体内快速的生物过程. It provides good optical sectioning capabilities and high speed with low levels of photobleaching.
   关键特性:
   • 4.200万像素CMOS摄像头.
   • 检测物镜:5倍、10倍、25倍水.
   • 双反射镜:2.5毫米、5毫米和7毫米.8 mm.
3. 共焦显微镜
   关键特性:
   • 紫外激光器405 nm，白光激光器WLL2 470-670 nm.
   • Detection objectives: 10x dry, 20x dry, 40x oil, 63X oil.
   • Five spectral detector channels: two PMTs and three HyDs.
   • 串联扫描器(8kHz).
   • XY扫描台.
   • HyVolution2超分辨率包

Leica's latest confocal system with three channel spectral detectors and HyVolution software that combines super-sensitive HyD detection with SVI Huygens deconvolution, allowing lateral resolution down to 140 nm compared with 200-250 nm for standard confocal microscopy.
关键特性:

• 紫外激光器405 nm, VIS激光器488nm, 552nm和638nm.
• 检测物镜:10倍干、20倍干、63倍油.
• Three spectral detector channels: two PMTs and one HyD.
• XY扫描台.
• HyVolution2超分辨率包.

操作规则

The Leica Microscopy core is open to all investigators in the University, we have to enforce some rules to insure every investigator has equal access to our equipment:

1. All users must be trained before they are allowed to use the microscopes. After training, an account will be set up for reserving time and using the microscope.
2. All users must sign up on the online calendar to reserve time before use of the microscopes.
3. 预订政策:
   1. 请不要提前2周以上预订
   2. 预约时间可分为两类:
      • 正常工作时段(星期一至五上午八时至下午五时)
      • Off-hour slots (weekend and weekdays before 8am or after 5pm).
      • During a regular working slot one user can reserve an instrument for max 4 hours per day and 8 hours per week; however this can be combined with an off-hour slot where the reservation is not limited.
   3. If you are unable to use the microscope that you have reserved, Please delete your reserved time on the calendar as far ahead as possible so others can see the time is available.
   4. If you finished before the end of your reserved time or cancel on the day of your reserved session, please inform the next or previous person in line so that he/she can start earlier or continue longer. If you are the last user of the day and you cancel your reserve within 24 hours, you are responsible for ensuring the microscope is turned off. Make sure to inform the user before you to shut down the system after finish, 否则他可能会把显微镜留给你. 
4. 使用显微镜:
   1. 请准时开始上课. 重复“不出席”将失去他们的注册特权.
   2. Please check the online booking calendar at the end of your session to facilitate last minute changes and scheduling updates. Leave the system on if someone is reserved next within two hours; keep the laser in standby whenever possible to prolong their life.
   3. Users must clean the microscope objectives they used and dispose of garbage when finish. If you bring any live specimens or potentially harmful samples, please dispose of them in your lab.
   4. The last user of the evening is responsible for shutting down the microscope system.
5. 你要对你的数据负责. 系统计算机用于临时图像存储. Please save your data on the data hard drive of the computer and transfer your image data as soon as possible after acquisition. Image files left on the computer hard drives will be removed in a period of time.
6. Users are required to report any problems or damages during usage of the microscope.
7. 显微镜室不允许携带食物或饮料.